CMV IgG Elisa Test Kit

INTENDED USE

CMV IgG Elisa test is intended for the detection and quantitative determination of IgG antibody to CMV in human sera.

ASSAY PROCEDURE

• Prepare Reagents: Dilute 1 volume of Washing Buffer (20×)with 19 volumes of distilled water, mix well.
• Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 100μL of Sample Diluent , dispense 10μL of sample, 100μL negative control or positive control to the respective wells(without Sample Diluent). Gently vibrating the plate.
• Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37°C for 30 minutes.
• Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
• Add Conjugate: Add 100μL of conjugate to each well (except the blank well).
• Incubate: Cover the Microplate and incubate the plate at 37°C for 30 minutes.
• Wash the Plate: Repeat the wash procedure as in step 4.
• Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37°C for 15 minutes.
• Stop reaction: Add 50μL Stop Solution to each well, mix well.
• Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

Storage

• Store at 2-8°C.

• Place unused wells in the zip-lock aluminum foiled pouch and return to 2-8 °C, under which conditions the wells will remain stable for 2 months, or until the labeled expiry date whichever is earlier.

• Seal and return unused calibrators to 2-8 °C, under which conditions the stability will be retained for 1 month, for longer use, store opened calibrators in aliquots and freeze at-20 °C. Avoid multiple freeze-thaw cycles.

• Seal and return all the other unused reagents to 2-8 °C, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.