Immunoassay for the in vitro quantitative determination of luteinizing hormone in
human serum.
Summary
LH (luteinizing hormone), together with FSH (follicle stimulating hormone),
belongs to the gonadotropin family. LH and FSH regulate and stimulate the growth
and function of the gonads (ovaries and testes) synergistically. Like FSH, TSH and
hCG, LH is a glycoprotein consisting of two subunits (α- and β-chains). (1, 2, 3)
This proteohormone, which consists of 121 amino acids and three sugar chains,
has a molecular weight of 29500 daltons. In women, the gonadotropins act within
the hypothalamus-pituitary-ovary regulating circuit to control the menstrual cycle.
(4, 5) LH and FSH are released in pulses from the gonadotropic cells of the
anterior pituitary and pass via the bloodstream to the ovaries. Here the
gonadotropins stimulate the growth and maturation of the follicle and hence the
biosynthesis of estrogens and progesterones. The highest LH-concentrations
occur during the mid-cycle peak and induce ovulation and formation of the corpus
luteum, the principal secretion product of which is progesterone. In the Leydig
cells of the testes, LH stimulates the production of testosterona. Determination of
the LH concentration is used in the elucidation of dysfunctions within the
hypothalamus-pituitary-gonads system. The determination of LH in conjunction
with FSH is utilized for the following indications: congenital diseases with
chromosome aberrations (e.g. Turner’s syndrome), polycystic ovaries (PCO),
clarifying the causes of amenorrhea, menopausal syndrome, and suspected
Leydig cell insufficiency. (1, 4, 5)
| Product |
Description |
| Specimen |
Serum |
| Format |
ELISA test |
| Kit Size96 Test/Kit |
96 tests/box |
| Manufacturer |
BIOVANTION |
| Exp |
18 months |
| Storage Conditions |
2-8℃ |
| Package |
carton |
| Application |
Be trying to get pregnant |
Features of Hbasg Elisa Kit:
1. Sensitive
2. Accurate3. High quality
4. Economic price
5. Reliable and Easy to Use
Test principle
Sandwich principle. Total duration of assay: 80 minutes.
• Sample, Anti-LH coated microwells and enzyme labeled Anti-LH are combined.
• During the incubation, LH presents in the sample is allowed to react
simultaneously with the two antibodies, resulting in the LH molecules being
sandwiched between the solid phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase, the LH within
the sample and enzyme-linked antibodies by immunological reactions.
• Substrate solution is then added and catalyzed by this complex, resulting in a
chromogenic reaction. The resulting chromogenic reaction is measured as
absorbance.
• The absorbance is proportional to the amount of LH in the sample.
Reagents
Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with mouse monoclonal
Anti-LH.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations: 0(A), 5(B), 20(C),
50(D), 100(E) and 200(F) mIU/mL.
• Enzyme Conjugate, 1 vial, 11 mL of HRP (horseradish peroxidase) labeled
mouse monoclonal Anti-LH in Tris-NaCl buffer containing BSA (bovine serum
albumin). Contains 0.1% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/L sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 mL (40X concentrated), PBS-Tween
wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
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